細(xì)胞內(nèi)鈣通量測(cè)定法是監(jiān)測(cè)GPCR和鈣通道活性的一種廣泛使用的方法。為了定量細(xì)胞內(nèi)鈣濃度，優(yōu)選比例熒光熒光指示劑，因?yàn)樵摫嚷逝c鈣濃度直接相關(guān)并且與細(xì)胞數(shù)和染料負(fù)載濃度無關(guān)。但是，流行的比例鈣指示劑（例如Fura-2和Indo-1）具有一定的局限性，例如靈敏度較低，紫外線激發(fā)，并且與HTS篩選濾光片組不兼容。Cal Red R525 / 650已開發(fā)為新型的488 nm可激發(fā)比例熒光鈣指示劑。Cal Red R525 / 650在488 nm處受到很好的激發(fā)，在525 nm和650 nm處有兩次發(fā)射。結(jié)合鈣后 當(dāng)在488 nm激發(fā)時(shí)，Cal Red R525 / 650的發(fā)射信號(hào)在525 nm處增加，在650 nm處降低。Cal Red R525 / 650的激發(fā)和發(fā)射波長(zhǎng)與普通濾光片組兼容，對(duì)細(xì)胞的損害小，使其成為評(píng)估和篩選GPCR激動(dòng)劑和拮抗劑以及鈣通道靶標(biāo)的強(qiáng)大工具

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