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Ex (nm)
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-
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Em (nm)
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-
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分子量
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~12000
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溶劑
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Water
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存儲(chǔ)條件
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在零下15度以下保存, 避免光照
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|
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產(chǎn)品基本信息
產(chǎn)品名稱：RatioWorks Cal-520/ zFluor 647 -葡聚糖綴合物* MW 10,000 *
儲(chǔ)存條件：-15℃避光防潮
保質(zhì)期：12個(gè)月
產(chǎn)品物理化學(xué)光譜特性
分子量：~12000
溶劑：水
鈣測(cè)量對(duì)于許多生物學(xué)研究至關(guān)重要。在結(jié)合鈣后顯示光譜響應(yīng)的熒光探針使研究人員能夠通過(guò)使用熒光顯微鏡，流式細(xì)胞儀，熒光光譜和熒光酶標(biāo)儀來(lái)研究細(xì)胞內(nèi)游離鈣濃度的變化?？梢允褂胮atch pipette或顯微注射將細(xì)胞不滲透葡聚糖的鈣指示劑物理加載到細(xì)胞上。使用熒光顯微鏡檢測(cè)來(lái)自這些細(xì)胞的熒光信號(hào)。與AM酯形式相比，我們的鈣指示劑的右旋糖酐形式很少出現(xiàn)在外漏和區(qū)室化的問(wèn)題。在熒光鈣指示劑右旋糖酐結(jié)合物中，Cal-520右旋糖酐結(jié)合物可能是好的選擇，因?yàn)樗鼈兊母邿晒饬孔赢a(chǎn)率和鈣的大量熒光增強(qiáng)作用。 RatioWorks Cal-520®/ zFluor 647 -右旋糖酐綴合物帶有不依賴鈣的iFluor 647熒光探針作為放射測(cè)量的參考色。 Cal-520熒光團(tuán)可以被488 nm的氬激光很好地激發(fā)，其中iFluor 647的激發(fā)少。參比iFluor 647熒光團(tuán)可以被633 nm的He-Ne激光或647 nm的紅色激光很好地激發(fā)，其中Cal-520熒光團(tuán)具有小的激發(fā)。與Cal-520L葡聚糖（＃20603）相比，此Cal-520葡聚糖對(duì)鈣離子具有更高的親和力，并且經(jīng)過(guò)優(yōu)化可監(jiān)測(cè)低水平的鈣離子。
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