鈣離子熒光探針Fluo-8L, 鈉鹽是美國AAT Bioquest生產(chǎn)的用于鈣通量測定的試劑，鈣測量對于許多生物學(xué)研究至關(guān)重要。在結(jié)合Ca2+后顯示光譜響應(yīng)的熒光探針使研究人員能夠使用熒光顯微鏡，流式細(xì)胞儀，熒光光譜和熒光酶標(biāo)儀來研究細(xì)胞內(nèi)游離Ca2+濃度的變化。在可見光激發(fā)鈣指示劑中，F(xiàn)luo-3和Fluo-4常用。但是，F(xiàn)luo-3 AM和Fluo-4 AM在酯酶水解后在活細(xì)胞中僅適度發(fā)熒光，并且需要苛刻的細(xì)胞加載條件才能大化其細(xì)胞鈣反應(yīng)。開發(fā)Fluo-8®染料可細(xì)胞負(fù)載和鈣響應(yīng)，同時(shí)保持便捷的Fluo-3和Fluo-4光譜波長（在?490 nm處具有大激發(fā)和在?520 nm處具有大發(fā)射）。Fluo-8®AM僅需要室溫，而Fluo-3 AM和Fluo-4 AM需要37℃的細(xì)胞負(fù)載。此外，F(xiàn)luo-8®的亮度是Fluo-4 AM的2倍，是Fluo-3 AM的4倍。AAT Bioquest提供了一套出色的Fluo-8®試劑，具有不同的鈣結(jié)合親和力（Fluo-8®：Kd = 389 nM； Fluo-8H：Kd = 232 nM； Fluo-8L：Kd = 1.86 μM； Fluo-8FF ：Kd = 10 μM）。百螢生物是AAT Bioquest 的中國代理商，為您提供的鈣離子熒光探針Fluo-8L, 鈉鹽

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