與Fluo-4等單波長探針不同，對于Ca2+，F(xiàn)ura探針的大吸收（或熒光激發(fā)）從380 nm（Fura-2），415 nm（Fura-8和Fura-10）轉(zhuǎn)變，與Ca2+結(jié)合的無螯合劑可達到約340 nm（Fura-2），355 nm（Fura-8和Fura-10），大熒光發(fā)射的波長相對獨立于Ca2+濃度。通過使用在340nm和380nm（對于Fura-2），355nm和415nm（對于Fura-8和Fura-10）進行激發(fā)并分配熒光強度來獲得Ca2+依賴性熒光信號的大動態(tài)范圍在?510 nm（Fura-2），525 nm（Fura-8和Fura-10）處檢測到。根據(jù)該比率，可以使用從校準曲線得出的解離常數(shù)（Kd）估算細胞內(nèi)Ca2+的水平。通過使用在兩個波長下激發(fā)產(chǎn)生的熒光強度之比，諸如染料分布不均勻和光致漂白等因素將被小化，因為它們應在相同程度上影響兩種測量。校準溶液初應不含重金屬離子，例如錳，這可能會影響其熒光和對鈣的親和力。百螢生物是AAT Bioquest的中國代理商，為您提供的Fura-10，鉀鹽。

參考文獻

A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells.
Authors: Lee, Jeong Hoon and Ha, Jeong Mi and Ho, Quynh Mai and Leem, Chae Hun
Journal: Journal of visualized experiments : JoVE (2019)

Calcium Imaging of Store-Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells Using Fura-2.
Authors: Johnson, Martin
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 163-172

Using FluoZin-3 and fura-2 to monitor acute accumulation of free intracellular Cd2+ in a pancreatic beta cell line.
Authors: Malaiyandi, Latha M and Sharthiya, Harsh and Barakat, Ameir N and Edwards, Joshua R and Dineley, Kirk E
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2019): 951-964

A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision.
Authors: Tinning, P W and Franssen, A J P M and Hridi, S U and Bushell, T J and McConnell, G
Journal: Journal of microscopy (2018): 212-220

Tuning the Spectroscopic Properties of Ratiometric Fluorescent Metal Indicators: Experimental and Computational Studies on Mag-fura-2 and Analogues.
Authors: Zhang, Guangqian and Jacquemin, Denis and Buccella, Daniela
Journal: The journal of physical chemistry. B (2017): 696-705